t175 cell culture flask (Sarstedt)

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Sarstedt t175 cell culture flask
T175 Cell Culture Flask, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t175+cell+culture+flasks/flasks+t75/pm42262667-115-16-20
Average 86 stars, based on 1 article reviews

t175 cell culture flask - by Bioz Stars, 2026-09

86/100 stars

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Cell Culture:

Article Title: Oxygen Supply of Islets of Langerhans by Photosynthetically Active Microalgae in Bioprinted Co-Cultures Maintains Their Function in a Hypoxic Environment.
Article Snippet: .. They were expanded in monolayer culture in T175 cell culture flasks (Sarstedt, Nümbrecht, Germany) at 37◦C and 5 % CO2 in a HERAcell CO2 incubator (Thermo Fisher Scientific, USA) with twice-weekly passaging. .. INS-1 were cultivated in RPMI-1640 medium with 11.1 mmol L−1 D-glucose (Gibco, Life Technologies, USA) supplemented with 10 % heat-inactivated fetal bovine serum (HI-FBS, Gibco), 100 U mL−1 penicillin and 100 μg mL−1 streptomycin (P/S; both Gibco), 10 mmol L−1 HEPES pH 7.4 (Carl Roth Germany), 2 mmol L−1 L-glutamine (Merck Millipore, Biochrom, Germany), 1 mmol L−1 sodium pyruvate (AppliChem, Germany), and 50 μmol L−1 2-mercaptoethanol (Sigma-Aldrich, Germany).

Article Title: Structural and Molecular Changes of Human Chondrocytes Exposed to the Rotating Wall Vessel Bioreactor.
Article Snippet: The commercially available immortalized human chondrocyte cell line C28/I2 (the cells were a kind gift from Mary Goldring) was cultured in complete medium (500 mL DMEM High Glucose (Sigma-Aldrich, St. Louis, MO, USA), FCS (10%), penicillin/streptomycin (1%), and sodium pyruvate (1%, 100 mM). .. The cells from frozen stocks were grown in T175 cell culture flasks (175 cm2; Sarstedt, Nümbrecht, Germany) for 3 days. ..

Article Title: Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.
Article Snippet: .. Five × 106 cells (NCH421k, NCH644, NCH705, and NCH711d) were seeded into each of 12 T175 cell culture flasks (Sarstedt, 83.3912.502) containing 25 mL of complete DMEM/Ham’s F12 medium and allowed to grow for 3 days. ..

Article Title: Oxygen Supply of Islets of Langerhans by Photosynthetically Active Microalgae in Bioprinted Co‐Cultures Maintains Their Function in a Hypoxic Environment
Article Snippet: .. They were expanded in monolayer culture in T175 cell culture flasks (Sarstedt, Nümbrecht, Germany) at 37°C and 5 % CO 2 in a HERAcell CO 2 incubator (Thermo Fisher Scientific, USA) with twice‐weekly passaging. .. INS‐1 were cultivated in RPMI‐1640 medium with 11.1 mmol L −1 D‐glucose (Gibco, Life Technologies, USA) supplemented with 10 % heat‐inactivated fetal bovine serum (HI‐FBS, Gibco), 100 U mL −1 penicillin and 100 μg mL −1 streptomycin (P/S; both Gibco), 10 mmol L −1 HEPES pH 7.4 (Carl Roth Germany), 2 mmol L −1 L‐glutamine (Merck Millipore, Biochrom, Germany), 1 mmol L −1 sodium pyruvate (AppliChem, Germany), and 50 μmol L −1 2‐mercaptoethanol (Sigma‐Aldrich, Germany).

Article Title: Osteogenic and angiogenic potential of molybdenum-containing mesoporous bioactive glass nanoparticles: An ionic approach to bone tissue engineering.
Article Snippet: .. Following isolation, the cells were cultured in T175 cell culture flasks (Sarstedt, Nümbrecht, Germany) coated with 0.1 % gelatin (Sigma-Aldrich, Steinheim, Germany). .. Expansion medium (EM) was used to grow the cells, consisting of Dulbecco’s modified Eagle’s medium (DMEM) high glucose, 12.5 % fetal calf serum (FCS), 2 mM L-glutamine, 1 % non-essential amino acids (NEAA), 50 μM β-mercaptoethanol (all Life Technologies, Darmstadt, Germany), 100 μg/ml penicillin/streptomycin (Sigma-Aldrich) and 4 ng/ml fibroblast growth factor 2 (Active Bioscience, Hamburg, Germany).

Article Title: Mesoporous bioactive glass nanoparticles exhibit intrinsic angiogenic potential in the chorioallantoic membrane assay, without the addition of exogenous cells.
Article Snippet: Angiogenesis is an essential part of bone regeneration, as neovascularisation supports the supply of necessary oxygen, nutrients, and cellular transport.. Bioactive glasses (BGs) have shown promise in enhancing both angiogenesis and bone regeneration.. For the evaluation of the angiogenic potential of BGs, the chorioallantoic membrane (CAM) assay constitutes an attractive experimental model and has already gained increasing attention in BG-focused research.

Article Title: Structural analysis of PLD3 reveals insights into the mechanism of lysosomal 5' exonuclease-mediated nucleic acid degradation.
Article Snippet: .. The stably transfected HEK293S GnTI - monoclonal cell line was raised in T175 cell culture flasks ( Sarstedt ) in growth medium ( DMEM containing 10% ( v / v ) fetal calf serum ) containing 7 μg / ml puromycin ( InvivoGen ) to ∼90% confluency, followed by the addition of production medium ( DMEM containing 2.5% ( v / v ) fetal calf serum ) for seven days. ..

Article Title: Estrogenic activity of E2-conjugated GLP-1 is mediated by intracellular endolysosomal acidification and estrone metabolism
Article Snippet: GLP-1-CEX, GLP-1-CEX/E2 and E2-3-ether were provided by Novo Nordisk (Indianapolis, Indiana, USA), and synthesized as previously published [ ]. hGLP-1 (7-36) amide was purchased from Anaspec (Cat #: AS22463, Fremont, CA, USA). .. HEK293T cells and MIN6 cells were cultured in T175 cell culture flasks (Sarstedt, Cat. No 83.3912.302). .. HEK293T cells were cultured in DMEM (Life Technologies, Cat. No 31966047) containing 10% heat-inactivated-foetal bovine serum (HI-FBS) (Gibco, Cat. No 10500064) and 1% Penicillin/Streptomycin (Pen/Strep) (Cat. No 5000956).

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t175 cell culture flask (Sarstedt)

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